W/o digestion, acid mucopolysaccharides and sialomucins - deep blue small bowel, appendix or colon for second control to demonstrate epithelial mucins Label slides one with w/o digestions one w/digestionĬontrol - umbilical cord (with and without). Staining will disappear or be dramtically reduced when tissue sections containing hyaluronic acid, chondroitin sulfate A or chondroitin sulfate C (CT "mucin") are digested w/ testicular hyaluronidase When used in a 0.1N hydrochloric acid solution (pH1.0) alcian blue stains only suflated acid mucopolysacchareds and sulfated sialomucins (glycogen)Īcid mucopolysaccharrides and sialomucins that are carboxylated only will not be stainedĬontrol - section of small intestine, appendix or colon (S.A.C.)ĭifferentiate epithelia and connective tissue mucins Weakly acidic sulfated mucosubstances, hyaluronic acid and sialomucins - dark blue When used in 3% acetic acid soluton (pH 2.5), alcian blue stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycogen)Ĭontrol section of small intestine, appendix or colon (S.A.C) repeat attempts to remove compound results in complete decolorizationįixatio in Carnoy solution will make acid-fast organisms non-acid-fastĪlcian blue is a copper phthalocyanin basic dye that is water soluble and colored blue bcuz ofits copper contentįorms salt linkages with the acid groups of acid mucopolysacchardes If section is allowed to dry after the carbol fuchsin, a compound that resist decolorization will be formed. If section is overstained take it back to the acid-alcohol to remove methylene blue, wash with water and repeat counterstaining step Overcountestaining w/methelyne blue will amsk any organisms present Negative control from same day work load must be runĪ section from a block of uterus provides good negative control Is more soluble in the lipids of the cell wall than in acid-alcohol, is readily removed from bacteria that lack waxy capsuleĪlthough 10% NBF is preferred, others m/b used, avoid carnoyĬontrol- tissue containing acid-fast organisms Lipoid capsule of acid-fast organism takes up carbol-fuchsin and resists decolorization w/dilute mineral acid Detects presence of acid-fast mycobacteria in tissue sections